Single bead parallel synthesis and screening

Vetter, D.; Thamm, A.; Schlingloff, G.; Schober, A.

doi:10.1023/A:1016287827310

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Single bead parallel synthesis and screening

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Thamm, A.
Department of Biochemistry, MPI for Chemical Ecology, Max Planck Society;

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Vetter, D., Thamm, A., Schlingloff, G., & Schober, A. (2000). Single bead parallel synthesis and screening. Molecular Diversity, 5(3), 111-116. doi:10.1023/A:1016287827310.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-B3F5-6

Abstract

Microstructured silicon wafers were employed as miniaturized solid-phase reaction vessels as well as miniaturized
micro titer plates. Employing piezoelectric drop-on-demand liquid jets, a combinatorial library of 256 Peptides
was synthesized on single beads. The synthesis protocol was associated to the location in the silicon nano-well
arrangement. Products were photolytically cleaved in the same well that was used for synthesis and subsequently
interrogated for thrombin inhibition in a homogeneous competition assay. The assay procedure was based on
drop-on-demand liquid delivery and laser induced fluorescence imaging. The novel format proved useful for the
integration of both synthesis and screening into one platform, a prerequisite for an iterative, evolutionary approach
towards drug discovery.