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Journal Article

Identification of auxin-binding proteins using an improved assay for photoaffinity labeling with tritiated 5-N-3-7 indole-3-acetic acid

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Citation

Campos, N., Feldwisch, J., Zettl, R., Boland, W., Schell, J., & Palme, K. (1991). Identification of auxin-binding proteins using an improved assay for photoaffinity labeling with tritiated 5-N-3-7 indole-3-acetic acid. Technique, 3(2), 69-75.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-9F8C-D
Abstract
We have developed an improved assay for detection of auxin-binding proteins using photoaffinity labeling. We have synthesized 5-NH2-[7-3H]indole-3-acetic acid (IAA) and 5-N3-[7-3H]IAA and extensively purified these compounds. Photoaffinity labeling of a