Direct quantification of dimethylsulfoniopropionate (DMSP) in marine micro- and 
macroalgae using HPLC or UPLC/MS

Wiesemeier, Theresa; Pohnert, Georg

doi:10.1016/j.jchromb.2006.12.023

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Direct quantification of dimethylsulfoniopropionate (DMSP) in marine micro- and macroalgae using HPLC or UPLC/MS

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Wiesemeier, T., & Pohnert, G. (2007). Direct quantification of dimethylsulfoniopropionate (DMSP) in marine micro- and macroalgae using HPLC or UPLC/MS. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 850(1-2), 493-498. doi:10.1016/j.jchromb.2006.12.023.

Cite as: https://hdl.handle.net/21.11116/0000-0003-4C4F-1

Abstract

A simple method for the direct quantification of dimethylsulfinopropionate (DMSP) using HPLC or UPLC coupled to UV and/or MS detection is introduced. The protocol is applied for the determination of DMSP from marine micro- and macroalgae. The method is based on the derivatisation of DMSP using 1-pyrenyidiazomethane followed by reversed phase HPLC or UPLC separation. The detection limit is 590 nM, corresponding to 1 ng DMSP per injection. Using a combination of UV and MS detection the calibration curves were linear in the range of 2.93 mu M to 11.7 mM concentrations. We show that direct determination of DMSP is possible from macroalgal tissue and microalgal cultures if DMSP-lyase activity is suppressed during work-up. (c) 2007 Elsevier B.V. All rights reserved.