Direct quantification of dimethylsulfoniopropionate (DMSP) with hydrophilic 
interaction liquid chromatography/mass spectrometry

Spielmeyer, Astrid; Pohnert, Georg

doi:10.1016/j.jchromb.2010.09.031

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Direct quantification of dimethylsulfoniopropionate (DMSP) with hydrophilic interaction liquid chromatography/mass spectrometry

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Spielmeyer, A., & Pohnert, G. (2010). Direct quantification of dimethylsulfoniopropionate (DMSP) with hydrophilic interaction liquid chromatography/mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 878(31), 3238-3242. doi:10.1016/j.jchromb.2010.09.031.

Cite as: https://hdl.handle.net/21.11116/0000-0003-51FC-6

Abstract

A simple derivatization free method for the direct determination of dimethylsulfoniopropionate (DMSP) using hydrophilic interaction liquid chromatography (HILIC)/mass spectrometry is Introduced DMSP is a zwitterionic osmolyte which is produced from marine plankton macro algae and higher plants Due to its central role in climate relevant geochemical processes as well as in plant physiology and chemical ecology there is a great interest in methods for its quantification Since DMSP is labile and difficult to extract currently most protocols for quantification are based on indirect methods Here we show that ultra performance liquid chromatography/mass spectrometry using a HILIC stationary phase is suitable for the direct quantification of DMSP from aqueous samples and microalgal extracts The protocol requires minimal sample preparation and phytoplankton samples can be investigated after filtration of small volumes The limit of detection is 20 nM and the calibration curve is linear in the range of 60 nM to 50 mu M The use of [H-2(6)]-DMSP as internal standard allows prolonged sample storage since it is transformed with the same kinetics as natural DMSP This makes the method suitable for both laboratory and field studies (C) 2010 Elsevier BV All rights reserved