Vacuole-targeted proteins: ins and outs of subcellular localization studies

Carqueijeiro, Inês; Sepúlveda, Liuda J.; Mosquera, Angela; Payne, Richard; Corbin, Cyrielle; Papon, Nicolas; de Bernonville, Thomas Dugé; Besseau, Sébastien; Lanoue, Arnaud; Glévarec, Gaëlle; Clastre, Marc; St-Pierre, Benoit; Atehortùa, Lucia; Giglioli-Guivarc’h, Nathalie; O'Connor, Sarah E.; Oudin, Audrey; Courdavault, and Vincent

doi:10.1007/978-1-4939-7856-4_4

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Book Chapter

Vacuole-targeted proteins: ins and outs of subcellular localization studies

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Carqueijeiro, I., Sepúlveda, L. J., Mosquera, A., Payne, R., Corbin, C., Papon, N., et al. (2018). Vacuole-targeted proteins: ins and outs of subcellular localization studies. In C. Pereira (Ed.), Plant Vacuolar Trafficking (pp. 33-54). New York City: Humania Press. doi:10.1007/978-1-4939-7856-4_4.

Cite as: https://hdl.handle.net/21.11116/0000-0002-D8EB-2

Abstract

Accurate and efficient demonstrations of protein localizations to the vacuole or tonoplast remain strict
prerequisites to decipher the role of vacuoles in the whole plant cell biology and notably in defence processes.
In this chapter, we describe a reliable procedure of protein subcellular localization study through
transient transformations of Catharanthus roseus or onion cells and expression of fusions with fluorescent proteins allowing minimizing artefacts of targeting.