Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule 
dynamics

Schellhaus, Anna Katharina; Moreno-Andres, Daniel; Chugh, Mayank; Yokoyama, Hideki; Moschopoulou, Athina; De, Suman; Bono, Fulvia; Hipp, Katharina; Schaeffer, Erik; Antonin, Wolfram

doi:10.1038/s41598-017-10088-5

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Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics

MPS-Authors

Schellhaus, Anna Katharina
Friedrich Miescher Laboratory, Max Planck Society;
Medical School, RWTH Aachen University;

Moreno-Andres, Daniel
Friedrich Miescher Laboratory, Max Planck Society;
Medical School, RWTH Aachen University;

Yokoyama, Hideki
Friedrich Miescher Laboratory, Max Planck Society;
Medical School, RWTH Aachen University;

Moschopoulou, Athina
Friedrich Miescher Laboratory, Max Planck Society;

Bono, Fulvia
Max Planck Institute for Developmental Biology, Max Planck Society;

Hipp, Katharina
Max Planck Institute for Developmental Biology, Max Planck Society;

Antonin, Wolfram
Friedrich Miescher Laboratory, Max Planck Society;
Medical School, RWTH Aachen University;

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https://www.nature.com/articles/s41598-017-10088-5
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Citation

Schellhaus, A. K., Moreno-Andres, D., Chugh, M., Yokoyama, H., Moschopoulou, A., De, S., et al. (2017). Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics. Scientific Reports, 7: 9996. doi:10.1038/s41598-017-10088-5.

Cite as: https://hdl.handle.net/21.11116/0000-0002-1487-F

Abstract

The mitotic spindle, essential for segregating the sister chromatids into the two evolving daughter cells, is composed of highly dynamic cytoskeletal filaments, the microtubules. The dynamics of microtubules are regulated by numerous microtubule associated proteins. We identify here Developmentally regulated GTP binding protein 1 (DRG1) as a microtubule binding protein with diverse microtubule-associated functions. In vitro, DRG1 can diffuse on microtubules, promote their polymerization, drive microtubule formation into bundles, and stabilize microtubules. HeLa cells with reduced DRG1 levels show delayed progression from prophase to anaphase because spindle formation is slowed down. To perform its microtubule-associated functions, DRG1, although being a GTPase, does not require GTP hydrolysis. However, all domains are required as truncated versions show none of the mentioned activities besides microtubule binding.