Targeted photoswitchable probe for nanoscopy of biological structures.

Dellagiacoma , C.; Lukinavicius, G.; Boccio, N.; Banala, S.; Geissbühler, S.; Märki, I.; Johnsson, K.; Lasser, T.

doi:10.1002/cbic.201000189

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Targeted photoswitchable probe for nanoscopy of biological structures.

MPS-Authors

/persons/resource/persons203920

Lukinavicius, G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

2628651_Suppl.pdf
(付録資料), 2MB

引用

Dellagiacoma , C., Lukinavicius, G., Boccio, N., Banala, S., Geissbühler, S., Märki, I., Johnsson, K., & Lasser, T. (2010). Targeted photoswitchable probe for nanoscopy of biological structures. ChemBioChem, 11(10), 1361-1363. doi:10.1002/cbic.201000189.

引用: https://hdl.handle.net/21.11116/0000-0001-DED7-3

要旨

SNAP‐tag: We introduce a photoswitchable O6‐benzylguanine derivative and demonstrate its use for super‐resolution microscopy of SNAP‐tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP‐tagged microtubule structures with ∼25 nm resolution. The described probe in combination with the versatile SNAP‐tag labeling opens new possibilities for imaging biological structures at the nanoscale.