Rational redesign of the ferredoxin-NADP+
-oxido-reductase/ferredoxin-interaction for photosynthesis-dependent H2
-production

Wiegand, K.; Winkler, M.; Rumpel, S.; Kannchen, D.; Rexroth, S.; Hase, T.; Farès, Christophe; Happe, T.; Lubitz, W.; Rögner, M.

doi:10.1016/j.bbabio.2018.01.006

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Rational redesign of the ferredoxin-NADP⁺-oxido-reductase/ferredoxin-interaction for photosynthesis-dependent H₂-production

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Farès, Christophe
Service Department Farès (NMR), Max-Planck-Institut für Kohlenforschung, Max Planck Society;

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Citation

Wiegand, K., Winkler, M., Rumpel, S., Kannchen, D., Rexroth, S., Hase, T., et al. (2018). Rational redesign of the ferredoxin-NADP⁺-oxido-reductase/ferredoxin-interaction for photosynthesis-dependent H₂-production. Biochimica et Biophysica Acta, Bioenergetics, 1859(4), 253-262. doi:10.1016/j.bbabio.2018.01.006.

Cite as: https://hdl.handle.net/21.11116/0000-0001-694B-6

Abstract

Utilization of electrons from the photosynthetic water splitting reaction for the generation of biofuels, commodities as well as application in biotransformations requires a partial rerouting of the photosynthetic electron transport chain. Due to its rather negative redox potential and its bifurcational function, ferredoxin at the acceptor side of Photosystem 1 is one of the focal points for such an engineering. With hydrogen production as model system, we show here the impact and potential of redox partner design involving ferredoxin (Fd), ferredoxin-oxido-reductase (FNR) and [FeFe]‑hydrogenase HydA1 on electron transport in a future cyanobacterial design cell of Synechocystis PCC 6803. X-ray-structure-based rational design and the allocation of specific interaction residues by NMR-analysis led to the construction of Fd- and FNR-mutants, which in appropriate combination enabled an about 18-fold enhanced electron flow from Fd to HydA1 (in competition with equimolar amounts of FNR) in in vitro assays. The negative impact of these mutations on the Fd-FNR electron transport which indirectly facilitates H₂ production (with a contribution of ≤42% by FNR variants and ≤23% by Fd-variants) and the direct positive impact on the Fd-HydA1 electron transport (≤23% by Fd-mutants) provide an excellent basis for the construction of a hydrogen-producing design cell and the study of photosynthetic efficiency-optimization with cyanobacteria.