Lysine-based amino-functionalized lipids for gene transfection: the influence 
of chain composition on 3D phase behaviour and transfection performance

Tassler, Stephanie; Pawlowska, Dorota; Janich, Christopher; Giselbrecht, Julia; Drescher, Simon; Langner, Andreas; Wolk, Christian; Brezesinski, Gerald

doi:10.1039/C8CP01922C

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Lysine-based amino-functionalized lipids for gene transfection: the influence of chain composition on 3D phase behaviour and transfection performance

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Tassler, Stephanie
Gerald Brezesinski, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Pawlowska, Dorota
Gerald Brezesinski, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Brezesinski, Gerald
Gerald Brezesinski, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Citation

Tassler, S., Pawlowska, D., Janich, C., Giselbrecht, J., Drescher, S., Langner, A., et al. (2018). Lysine-based amino-functionalized lipids for gene transfection: the influence of chain composition on 3D phase behaviour and transfection performance. Physical Chemistry Chemical Physics, 20(25), 17393-17405. doi:10.1039/C8CP01922C.

Cite as: https://hdl.handle.net/21.11116/0000-0001-60F3-0

Abstract

Based on previous work, the influence of the chain composition on the physical-chemical properties of five new transfection lipids (TH10, TT10, OH10, OT10, OO10) containing the same lysine-based head group has been investigated in aqueous dispersions. For this purpose, the chain composition has been gradually varied from saturated tetradecyl (T, C14:0) and hexadecyl (H, C16:0) chains to longer but unsaturated oleyl (O, C18:1) chains with double bonds in cis configuration. In this work, the lipid dispersions have been investigated in absence and presence of the helper lipid DOPE and calf thymus DNA by small-angle and wide-angle X-ray scattering (SAXS/WAXS) supplemented by differential scanning calorimetry (DSC), attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and Fourier transform Raman spectroscopy (FTRS). Lamellar and inverted hexagonal mesophases have been observed in single-component systems. In the binary mixtures, the aggregation behaviour changes with increasing amount of DOPE from lamellar to cubic. The lipid mixtures with DNA show panoply of mesophases. Interestingly, TT10 and OT10 form cubic lipoplexes, whereas OO10 complexes the DNA sandwich-like between lipid bilayers in a lamellar lipoplex. Surprisingly, the latter one is the most effective lipoplex.