English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Structure of genes for human growth hormone and chorionic somatomammotropin

MPS-Authors
/persons/resource/persons95292

Seeburg,  Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://www.pnas.org/content/76/9/4294.full.pdf
(Any fulltext)

https://doi.org/10.1073/pnas.76.9.4294
(Any fulltext)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Fiddes, J. C., Seeburg, P. H., DeNoto, F. M., Hallewell, R. A., Baxter, J. D., & Goodman, H. M. (1979). Structure of genes for human growth hormone and chorionic somatomammotropin. Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4294-4298. doi:10.1073/pnas.76.9.4294.


Cite as: https://hdl.handle.net/21.11116/0000-0001-2473-5
Abstract
A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences have been identified by hybridization to cloned cDNA. Human DNA was cleaved with EcoRI and fractionated by preparative agarose gel electrophoresis; DNA in the size range 2--3 kb was ligated to lambda gt WES.lambda B DNA and viable recombinant bacteriophage were recovered by in vitro packaging. After infection of Escherichia coli and screening of phage plaques, single isolates of hGH and hCS gene sequences were obtained. Restriction endonuclease mapping showed that the hGH gene contains three intervening sequences interrupting the coding sequence. Partial DNA sequence analysis of the hGH gene, obtained by the chain termination method, confirmed the location of the intervening sequences and the identity of the fragment.