Resolution in Electron Tomography

Kudryashev, Misha

doi:https://doi.org/10.1007/978-3-319-68997-5_10

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Resolution in Electron Tomography

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Kudryashev, Misha
Sofja Kovalevskaja Group, Max Planck Institute of Biophysics, Max Planck Society;
Buchmann Institute for Molecular Life Sciences, Goethe University of Frankfurt am Main;

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Citation

Kudryashev, M. (2018). Resolution in Electron Tomography. In E. Hanson (Ed.), Biological and Medical Physics, Biomedical Engineering (BIOMEDICAL) (pp. 261-282 ). 6330 Cham Switzerland: Springer International Publishing AG 2018. doi:https://doi.org/10.1007/978-3-319-68997-5_10.

Cite as: https://hdl.handle.net/21.11116/0000-0001-278F-3

Abstract

Electron microscopes yield point resolution on the order of one angstrom, however the density maps from electron tomography typically have resolutions in the nanometre range. In this chapter I qualitatively discuss the typical limitations that occur in electron tomography of biological samples depending on the imaging modalities, with the focus on cryo electron tomography and subtomogram averaging