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Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans

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Hannak,  E.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Kirkham,  M.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Hyman,  A. A.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219500

Oegema,  K.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Hannak, E., Kirkham, M., Hyman, A. A., & Oegema, K. (2001). Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans. Journal of Cell Biology, 155(7), 1109-1115.


Cite as: https://hdl.handle.net/21.11116/0000-0001-13E6-6
Abstract
Centrosomes mature as cells enter mitosis, accumulating gamma - tubulin and other pericentriolar material (PCM) components. This occurs concomitant with an increase in the number of centrosomally organized microtubules (MTs). Here, we use RNA- mediated interference (RNAi) to examine the role of the aurora- A kinase, AlR-1, during centrosome maturation in Caenorhabditis elegans. In air-1(RNAi) embryos, centrosomes separate normally, an event that occurs before maturation in C. elegans. After nuclear envelope breakdown, the separated centrosomes collapse together, and spindle assembly fails. In mitotic air-1(RNAi) embryos, centrosomal alpha -tubulin fluorescence intensity accumulates to only 40% of wild-type levels, suggesting a defect in the maturation process. Consistent with this hypothesis, we find that AIR-1 is required for the increase in centrosomal gamma -tubulin and two other PCM components, ZYG-9 and CeGrip, as embryos enter mitosis. Furthermore, the AIR-1- dependent increase in centrosomal gamma -tubulin does not require MTs. These results suggest that aurora-A kinases are required to execute a MT-independent pathway for the recruitment of PCNA during centrosome maturation.