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Gene expression in Acetabularia. II. Analysis of in vitro translation products

MPG-Autoren
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Shoeman,  Robert L.
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society;

Externe Ressourcen

http://jcs.biologists.org/content/58/1/35.full.pdf
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Zitation

Shoeman, R. L., & Schweiger, H. (1982). Gene expression in Acetabularia. II. Analysis of in vitro translation products. Journal of Cell Science, 58(1), 35-48. Retrieved from http://jcs.biologists.org/content/58/1/35.


Zitierlink: https://hdl.handle.net/21.11116/0000-0000-E8E0-D
Zusammenfassung
The translation products induced by poly(A)+ RNA from Acetabularia mediterranea, A. cliftonii and A. ryukyuensis in a modified, highly efficient wheat germ cell-free in vitro system were separated by two-dimensional gel electrophoresis. A comparison of the translation products on the basis of their molecular weight and their isoelectric point revealed only a limited similarity between the patterns of the three species. The pronounced species specificity will permit the study of the in vivo translation of heterologous poly(A)+ RNA in Acetabularia cytoplasm.