Human antisera detect a Plasmodium falciparum genomic clone encoding a 
nonapeptide repeat

Koenen, Michael; Scherf, Artur; Mercereau−Puijalon, Odile; Langsley, Gordon; Sibilli, Lisa; Dubois, Philippe; Pereira da Silva, Luis; Müller−Hill, Benno

doi:10.1038/311382a0

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Human antisera detect a Plasmodium falciparum genomic clone encoding a nonapeptide repeat

MPS-Authors

/persons/resource/persons93829

Koenen, Michael
Molecular anatomy of the neuromuscular junction, Max Planck Institute for Medical Research, Max Planck Society;
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Working Group Witzemann / Koenen, Max Planck Institute for Medical Research, Max Planck Society;
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.nature.com/articles/311382a0.pdf
(Any fulltext)

https://doi.org/10.1038/311382a0
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

Nature_311_1984_382.pdf
(Any fulltext), 545KB

Supplementary Material (public)

There is no public supplementary material available

Citation

Koenen, M., Scherf, A., Mercereau−Puijalon, O., Langsley, G., Sibilli, L., Dubois, P., et al. (1984). Human antisera detect a Plasmodium falciparum genomic clone encoding a nonapeptide repeat. Nature, 311(5984), 382-385. doi:10.1038/311382a0.

Cite as: https://hdl.handle.net/21.11116/0000-0000-D3D2-4

Abstract

Plasmodium falciparum causes malaria infections in its human host. Its wide distribution in tropical countries is a major world health problem. Before a vaccine can be produced, the identification and characterization of parasite antigens is necessary. This can be achieved by the cloning and subsequent analysis of genes coding for parasite antigens. Recently established cDNA banks allow the expression of cDNA derived from the simian parasite Plasmodium knowlesi and P. falciparum in Escherichia coli. Recombinants encoding parasite antigens have been identified by immunodetection in both banks. Two of them contain repetitive units of 11 (ref. 7) or 12 (ref. 5) amino acids. We describe here the construction of an expression bank made directly from randomly generated fragments of P. falciparum genomic DNA. We detect several clones which react strongly with human African immune sera. One clone expresses an antigenic determinant composed of occasionally degenerated repeats of a peptide nonamer.