The 43-K protein, v1, associated with acetylcholine receptor containing 
membrane fragments is an actin-binding protein

Walker, J. H.; Boustead, Catherine; Witzemann, Veit

doi:10.1002/j.1460-2075.1984.tb02127.x

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The 43-K protein, v1, associated with acetylcholine receptor containing membrane fragments is an actin-binding protein

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Witzemann, Veit
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Working Group Witzemann / Koenen, Max Planck Institute for Medical Research, Max Planck Society;
Molecular anatomy of the neuromuscular junction, Max Planck Institute for Medical Research, Max Planck Society;
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Walker, J. H., Boustead, C., & Witzemann, V. (1984). The 43-K protein, v1, associated with acetylcholine receptor containing membrane fragments is an actin-binding protein. EMBO Journal, 3(10), 2287-2290. doi:10.1002/j.1460-2075.1984.tb02127.x.

Cite as: https://hdl.handle.net/21.11116/0000-0000-D3CA-E

Abstract

Acetylcholine receptor enriched membrane fragments were obtained from the electric organs of Torpedo marmorata. The purified membrane fragments contained several proteins in addition to the acetylcholine receptor subunits. One of these was shown to be actin by means of immune blotting with a monoclonal antibody. Brief treatment of the membranes with pH 11.0 buffer removed actin and the other non-receptor proteins including the receptor-associated 43 000 mol. wt. polypeptide. This polypeptide was shown to bind actin after transferring the proteins from one- and two-dimensional polyacrylamide gels to nitrocellulose paper and incubating the nitrocellulose blots with actin. Specifically bound actin was demonstrated using the monoclonal antibodies to actin. No calcium or calmodulin dependency of binding was observed. The findings suggest that the 43 000 mol. wt. polypeptide is a link between the membrane-bound acetylcholine receptor and the cytoskeleton.