Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene 
product and S-adenosylmethionine on the expression of the metF gene

Shoeman, Robert L.; Redfield, Betty; Coleman, Timothy; Greene, Ronald C.; Smith, Albert A.; Brot, Nathan; Weissbach, Herbert

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Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene product and S-adenosylmethionine on the expression of the metF gene

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Shoeman, Robert L.
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Shoeman, R. L., Redfield, B., Coleman, T., Greene, R. C., Smith, A. A., Brot, N., et al. (1985). Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene product and S-adenosylmethionine on the expression of the metF gene. Proceedings of the National Academy of Sciences of the United States of America, 82(11), 3601-3605. Retrieved from http://www.pnas.org/content/82/11/3601.

Cite as: https://hdl.handle.net/21.11116/0000-0000-CF11-4

Abstract

The regulation of the expression of the Escherichia coli metF gene, which codes for 5,10-methylenetet-rahydrofolate reductase (EC 1.1.99.15), has been investigated by using a simplified DNA-directed in vitro system that measures the formation of the first dipeptide (fMet-Ser) of the gene product. The synthesis of fMet-Ser directed by a plasmid containing the metF gene is specifically inhibited by metJ protein (repressor protein). S-Adenosylmethionine enhances the inhibition by the metJ protein of metF gene expression. The inhibition by the metJ protein is at the level of transcription and the results suggest that S-adenosylmethionine is functioning as an allosteric effector.