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Journal Article

Long-term, noninvasive imaging of regulated gene expression in living mice

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Hasan,  Mazahir T.
Mazahir Hasan Group, Max Planck Institute for Medical Research, Max Planck Society;
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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http://onlinelibrary.wiley.com/doi/10.1002/gene.1014/epdf
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https://doi.org/10.1002/gene.1014
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Citation

Hasan, M. T., Schönig, K., Berger, S., Graewe, W., & Bujard, H. (2001). Long-term, noninvasive imaging of regulated gene expression in living mice. Genesis, 29(3), 116-122. doi:10.1002/gene.1014.


Cite as: https://hdl.handle.net/21.11116/0000-0000-3F00-A
Abstract
We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long-term studies of gene function in individual mice.