The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis.

Lorenzi, I.; Oeljeklaus, S.; Aich, A.; Ronsör, C.; Callegari, S.; Dudek, J.; Warscheid, B.; Dennerlein, S.; Rehling, P.

doi:10.1016/j.bbamcr.2017.11.010

Item

ITEM ACTIONSEXPORT

Add to Basket

Please note that a newer version of this item is available:
https://pure.mpg.de/pubman/item/item_2504412_6

DetailsSummary

Released

Journal Article

The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis.

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

2504412.pdf
(Publisher version), 915KB

Supplementary Material (public)

There is no public supplementary material available

Citation

Lorenzi, I., Oeljeklaus, S., Aich, A., Ronsör, C., Callegari, S., Dudek, J., et al. (2017). The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1865(2), 323-333. doi:10.1016/j.bbamcr.2017.11.010.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-37BA-A

Abstract

The three mitochondrial-encoded proteins, COX1, COX2, and COX3, form the core of the cytochrome c oxidase. Upon synthesis, COX2 engages with COX20 in the inner mitochondrial membrane, a scaffold protein that recruits metallochaperones for copper delivery to the CuA-Site of COX2. Here we identified the human protein, TMEM177 as a constituent of the COX20 interaction network. Loss or increase in the amount of TMEM177 affects COX20 abundance leading to reduced or increased COX20 levels respectively. TMEM177 associates with newly synthesized COX2 and SCO2 in a COX20-dependent manner. Our data shows that by unbalancing the amount of TMEM177, newly synthesized COX2 accumulates in a COX20-associated state. We conclude that TMEM177 promotes assembly of COX2 at the level of CuA-site formation.