Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
  DetailsÜbersicht

Freigegeben
Zeitschriftenartikel

Both Nodal signalling and stochasticity select for prospective distal visceral endoderm in mouse embryos.

MPG-Autoren
/persons/resource/persons204665

Takaoka,  K.
Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society;

Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)

2503041.pdf
(Verlagsversion), 4MB

Ergänzendes Material (frei zugänglich)

2503041_Suppl_1.pdf
(Ergänzendes Material), 29MB

2503041_Suppl_2.pdf
(Ergänzendes Material), 17MB

2503041_Suppl_3.docx
(Ergänzendes Material), 16KB

2503041_Suppl_4.avi
(Ergänzendes Material), 4MB

2503041_Suppl_5.avi
(Ergänzendes Material), 3MB

2503041_Suppl_6.avi
(Ergänzendes Material), 2MB

Zitation

Takaoka, K., Nishimura, H., & Hamada, H. (2017). Both Nodal signalling and stochasticity select for prospective distal visceral endoderm in mouse embryos. Nature Communications, 8: 1492. doi:10.1038/s41467-017-01625-x.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002E-3116-0
Zusammenfassung
Anterior-posterior (A-P) polarity of mouse embryos is established by distal visceral endoderm (DVE) at embryonic day (E) 5.5. Lefty1 is expressed first at E3.5 in a subset of epiblast progenitor cells (L1epi cells) and then in a subset of primitive endoderm cells (L1dve cells) fated to become DVE. Here we studied how prospective DVE cells are selected. Lefty1 expression in L1epi and L1dve cells depends on Nodal signaling. A cell that experiences the highest level of Nodal signaling begins to express Lefty1 and becomes an L1epi cell. Deletion of Lefty1 alone or together with Lefty2 increased the number of prospective DVE cells. Ablation of L1epi or L1dve cells triggered Lefty1 expression in a subset of remaining cells. Our results suggest that selection of prospective DVE cells is both random and regulated, and that a fixed prepattern for the A-P axis does not exist before the blastocyst stage.