Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging

Smith, Gordon B.; Fitzpatrick, David

doi:10.1007/978-1-4939-6352-2_10

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Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging

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Smith, Gordon B.
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Fitzpatrick, David
Max Planck Florida Institute for Neuroscience, Max Planck Society;

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Citation

Smith, G. B., & Fitzpatrick, D. (2016). Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging. Methods in Molecular Biology (Clifton, N.J.), 1474, 171-185. doi:10.1007/978-1-4939-6352-2_10.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-2AD6-2

Abstract

Two-photon (2P) imaging has proven to be a powerful tool for investigating neural structure and function both in brain slices and in intact systems. In vivo 2P imaging presents significant challenges in sample preparation, which are exacerbated in non-murine species. Here, we describe procedures for the effective virally mediated labeling of neurons and for the implantation of cranial windows for imaging. The procedures described here are applicable to a range of species, including mice, and are routinely used in ferrets and tree shrews to provide large-scale labeling of cortical volumes and high-quality imaging data.