A tunable refractive index matching medium for live imaging cells, tissues and 
model organisms

Boothe , Tobias; Hilbert, Lennart; Heide, Michael; Berninger, Lea; Huttner, Wieland B.; Zaburdaev, Vasily; Vastenhouw, Nadine L.; Myers, Eugene W.; Drechsel, David N.; Rink, Jochen C.

doi:10.7554/eLife.27240

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A tunable refractive index matching medium for live imaging cells, tissues and model organisms

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Hilbert, Lennart
Max Planck Institute for the Physics of Complex Systems, Max Planck Society;

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Zaburdaev, Vasily
Max Planck Institute for the Physics of Complex Systems, Max Planck Society;

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https://elifesciences.org/articles/27240
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Citation

Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W. B., Zaburdaev, V., et al. (2017). A tunable refractive index matching medium for live imaging cells, tissues and model organisms. eLife, 6: e27240. doi:10.7554/eLife.27240.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-2808-2

Abstract

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.