Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and 
fluorescent modified nucleotides.

Carrocci, T. J.; Lohe, L.; Ashton, M. J.; Höbartner, C.; Hoskins, A. A.

doi:10.1039/c7cc06703h

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Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides.

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Höbartner, C.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

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Citation

Carrocci, T. J., Lohe, L., Ashton, M. J., Höbartner, C., & Hoskins, A. A. (2017). Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides. Chemical Communications, 53(88), 11992-11995. doi:10.1039/c7cc06703h.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-198D-2

Abstract

The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2′,5′-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.