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Book Chapter

Analysis of Yeast Mitochondria by Electron Microscopy

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Neupert,  Walter
Neupert, Walter / Structure and Function of Mitochondria, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Unger, A.-K., Geimer, S., Harner, M., Neupert, W., & Westermann, B. (2017). Analysis of Yeast Mitochondria by Electron Microscopy. In D. Mokranjac, & F. Perocchi (Eds.), Mitochondria (2nd ed., pp. 293-314). New York, NY: Humana Press.


Cite as: https://hdl.handle.net/21.11116/0000-0000-7807-2
Abstract
Budding yeast Saccharomyces cerevisiae represents a widely used model organism for the study of mitochondrial biogenesis and architecture. Electron microscopy is an essential tool in the analysis of cellular ultrastructure and the precise localization of proteins to organellar subcompartments. We provide here detailed protocols for the analysis of yeast mitochondria by transmission electron microscopy: (1) chemical fixation and Epon embedding of yeast cells and isolated mitochondria, and (2) cryosectioning and immunolabeling of yeast cells and isolated mitochondria according to the Tokuyasu method.