English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Thiophosphate-Analogues and 1-N-Oxides of ATP and ADP in Mitochondrial Translocation and Phosphoryl-Transfer Reactions

MPS-Authors
/persons/resource/persons182136

Eckstein,  Fritz
Max Planck Institute of Experimental Medicine, Max Planck Society;

/persons/resource/persons98693

Goody,  Roger S.
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Schlimme, E., Lambrecht, W., Eckstein, F., & Goody, R. S. (1973). Thiophosphate-Analogues and 1-N-Oxides of ATP and ADP in Mitochondrial Translocation and Phosphoryl-Transfer Reactions. European Journal of Biochemistry, 40(2), 485-491. doi:10.1111/j.1432-1033.1973.tb03217.x.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-DC93-F
Abstract
1Various thiophosphate analogues of adenine nucleotides, such as adenosine 5′-O-(1-thiotriphosphate), adenosine 5′-O-(3-thiotriphosphate), adenosine 5′-O-(1-thiodiphosphate) and adenosine 5′-O-(2-thiodiphosphate) as well as the 1-N-oxides of adenosine 5′-O-tri(and-di)-phosphates show atractyloside-sensitive (i.e. adenine nucleotide-“carrier”-linked) and insensitive (non-“carrier”-linked) binding properties to rat liver mitochondria. 2Modified nucleoside diphosphates, such as adenosine 5′-O-(1-thiodiphosphate) and adenosine 5′-O-(2-thiodiphosphate) do not react with adenosine 5′-O-triphosphate synthetase (ATP-synthetase) in oxidative phosphorylation. Both are potent inhibitors of mitochondrial state-3 respiration whereas the 1-N-oxide of adenosine 5′-O-diphosphate does not interfere. 3Substitution of phosphate-moiety oxygen by sulfur affects enzymatic γ-phosphoryltransfer reactions. Adenosine 5′-O-(1-thiodiphosphate) is a substrate for nucleside diphosphate kinase whereas adenosine 5′-O-(2-thiodiphosphate) is not. Adenosine 5′-O-(1-thiotriphosphate) but not adenosine 5′-O-(3-thiotriphosphate) can replace adenosine 5′-O-triphosphate in hexokinase-catalyzed glucose-phosphorylation.