Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Cellular Prion Protein PrPC and Ecto-5 '-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy

MPG-Autoren
/persons/resource/persons183407

Seget,  Katarzyna
Storchova, Zuzana / Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78761

Storchova,  Zuzana
Storchova, Zuzana / Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Max Planck Society;

Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte in PuRe verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Domingues, P. H., Nanduri, L. S. Y., Seget, K., Venkateswaran, S. V., Agorku, D., Vigano, C., et al. (2017). Cellular Prion Protein PrPC and Ecto-5 '-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy. Cancer research: an official organ of the American Association for Cancer Research, 77(11), 2914-2926. doi:10.1158/0008-5472.CAN-16-3052.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002D-DE7C-A
Zusammenfassung
Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of approximately 300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples, and mouse models. Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrPC and the immunosuppressive cell surface enzyme ecto-5'-nucleotidase CD73. Functional analyses associated these alterations with increased cellular stress. An increased number of CD73(+) cells was observed in confluent cultures in aneuploid cells relative to their diploid counterparts. An elevated expression in CD230/PrPC was observed in serum-deprived cells in association with increased generation of reactive oxygen species. Overall, our work identified biomarkers of aneuploid karyotypes, which suggest insights into the underlying molecular physiology of aneuploid cells. (C) 2017 AACR.