Analysis of herbivore stress- and phytohormone-mediated urease expression in 
soybean (Glycine max)

Menegassi, A.; Da Silva e Silva, R.; Carlini, C. R.; Mithöfer, Axel; Becker-Ritt, A. B.

doi:10.1007/s00344-017-9739-x

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Analysis of herbivore stress- and phytohormone-mediated urease expression in soybean (Glycine max)

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Mithöfer, Axel
Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;
Research Group Dr. A. Mithöfer, Plant Defense Physiology, Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;

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Citation

Menegassi, A., Da Silva e Silva, R., Carlini, C. R., Mithöfer, A., & Becker-Ritt, A. B. (2018). Analysis of herbivore stress- and phytohormone-mediated urease expression in soybean (Glycine max). Journal of Plant Growth Regulation, 37(2), 419-425. doi:10.1007/s00344-017-9739-x.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-C36B-9

Abstract

Ureases catalyze the hydrolysis of urea into
ammonia and carbon dioxide and, thus, are involved in the
metabolism and bioavailability of nitrogen. Ureases occur in
plants, fungi, and bacteria. In plants, besides their enzymatic
activity, ureases as proteins play a role in defense against
insect and phytopathogenic fungi. Little is known about the
regulation of urease in plants under stress and whether or not
phytohormones may be involved. In this study, we addressed
the regulation of ubiquitous urease (ubSBU) gene expression
after phytohormone applications, insect herbivory, and
mechanical damage in soybean (Glycine max cv. Williams
82). Stress-related phytohormones were applied. In addition,
Spodoptera littoralis feeding and mechanical damage
by MecWorm were performed. Ureolytic activity and
transcripts for ubSBU and UreG were quantified. Roots and
leaves showed the highest levels of ubSBU transcripts. The results show a significant increase of ubSBU transcripts upon
jasmonic acid application and after herbivory, but downregulation
after MecWorm treatment. UreG transcripts were
downregulated after MecWorm, S. littoralis, and application
of gibberellic acid, but upregulated by jasmonic acid.
However, the ureolytic activities in leaves were influenced
neither by phytohormones nor by herbivory and MecWorm.
We conclude that the enzymatic activity of ureases is constitutive
and basal levels of the enzyme are sufficient to perform
the ureolytic activities in defense against insects and
fungi. The defense role of ureases, which does not require
the ureolytic activity, may underlie their differential regulation
in response to different stress stimuli.