EpCAM controls morphogenetic programs during zebrafish pronephros development

Kuechlin, Sebastian; Schoels, Maximilian; Slanchev, Krasimir; Lassmann, Silke; Walz, Gerd; Yakulov, Toma A.

doi:10.1016/j.bbrc.2017.04.035

Item

ITEM ACTIONSEXPORT

Add to Basket

Please note that a newer version of this item is available:
https://pure.mpg.de/pubman/item/item_2464949_4

DetailsSummary

Released

Journal Article

EpCAM controls morphogenetic programs during zebrafish pronephros development

MPS-Authors

/persons/resource/persons130715

Slanchev, Krasimir
Department: Genes-Circuits-Behavior / Baier, MPI of Neurobiology, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Kuechlin, S., Schoels, M., Slanchev, K., Lassmann, S., Walz, G., & Yakulov, T. A. (2017). EpCAM controls morphogenetic programs during zebrafish pronephros development. Biochemical and Biophysical Research Communications, 487(2), 209-215. doi:10.1016/j.bbrc.2017.04.035.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-A506-A

Abstract

Epithelial cell adhesion molecule EpCAM is a transmembrane glycoprotein that is dynamically expressed in human and murine renal epithelia during development. The levels of EpCAM in the renal epithelium are upregulated both during regeneration after ischemia/reperfusion injury and in renal-derived carcinomas. The role of EpCAM in early kidney development, however, has remained unclear. The zebrafish pronephros shows a similar segmentation pattern to the mammalian metanephric nephron, and has recently emerged as a tractable model to study the regulatory programs governing early nephrogenesis. Since EpCAM shows persistent expression in the pronephros throughout early development, we developed a method to study the global changes in gene expression in specific pronephric segments of wild type and EpCAM-deficient zebrafish embryos. In epcam mutants, we found 379 differentially expressed genes. Gene ontology analysis revealed that EpCAM controls various developmental programs, including uretric bud development, morphogenesis of branching epithelium, regulation of cell differentiation and cilium morphogenesis. (C) 2017 Elsevier Inc. All rights reserved.