Glycan Fingerprinting using Cold-Ion Infrared Spectroscopy

Mucha, Eike; Gonzalez Florez, Ana Isabel; Marianski, Mateusz; Thomas, Daniel; Hoffmann, Waldemar; Struwe, Weston B.; Hahm, Heung Sik; Gewinner, Sandy; Schöllkopf, Wieland; Seeberger, Peter H.; Helden, Gert von; Pagel, Kevin

doi:10.1002/anie.201702896

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Glycan Fingerprinting using Cold-Ion Infrared Spectroscopy

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Mucha, Eike
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institut für Chemie und Biochemie der Freien Universität Berlin;

/persons/resource/persons21566

Gonzalez Florez, Ana Isabel
Molecular Physics, Fritz Haber Institute, Max Planck Society;

/persons/resource/persons104335

Marianski, Mateusz
Molecular Physics, Fritz Haber Institute, Max Planck Society;

/persons/resource/persons203721

Thomas, Daniel
Molecular Physics, Fritz Haber Institute, Max Planck Society;

/persons/resource/persons59166

Hoffmann, Waldemar
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institut für Chemie und Biochemie der Freien Universität Berlin;

/persons/resource/persons121376

Hahm, Heung Sik
Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons21548

Gewinner, Sandy
Molecular Physics, Fritz Haber Institute, Max Planck Society;

/persons/resource/persons22079

Schöllkopf, Wieland
Molecular Physics, Fritz Haber Institute, Max Planck Society;

/persons/resource/persons121849

Seeberger, Peter H.
Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;
Institut für Chemie und Biochemie der Freien Universität Berlin;

/persons/resource/persons21614

Helden, Gert von
Molecular Physics, Fritz Haber Institute, Max Planck Society;

/persons/resource/persons32738

Pagel, Kevin
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institut für Chemie und Biochemie der Freien Universität Berlin;

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Citation

Mucha, E., Gonzalez Florez, A. I., Marianski, M., Thomas, D., Hoffmann, W., Struwe, W. B., et al. (2017). Glycan Fingerprinting using Cold-Ion Infrared Spectroscopy. Angewandte Chemie International Edition. doi:10.1002/anie.201702896.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-4DB3-9

Abstract

The diversity of stereochemical isomers present in glycans and glycoconjugates poses a formidable challenge for comprehensive structural analysis. Typically, sophisticated mass spectrometry (MS)- based techniques are used in combination with chromatography or ion mobility separation. However, coexisting structurally similar isomers often render an unambiguous identification impossible. Other powerful techniques such as gas-phase infrared (IR) spectroscopy have been limited to smaller glycans, as conformational flexibility and thermal activation during the measurement result in poor spectral resolution. Here, we show that this limitation can be overcome using cold-ion spectroscopy. The vibrational fingerprints of cold oligosaccharide ions exhibit a wealth of well-resolved absorption features that are diagnostic for minute structural variations. The unprecedented resolution of cold-ion spectroscopy coupled with tandem MS may render this the key technology to unravel complex glycomes.