Automated glycan assembly using the Glyconeer 2.1 synthesizer

Hahm, Heung Sik; Schlegel, Mark K.; Hurevich, Mattan; Eller, Steffen; Schuhmacher, Frank; Hofmann, Johanna; Pagel, Kevin; Seeberger, Peter H.

doi:10.1073/pnas.1700141114

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Automated glycan assembly using the Glyconeer 2.1 synthesizer

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Hahm, Heung Sik
Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;
Institute of Chemistry and Biochemistry, Free University Berlin;

/persons/resource/persons121819

Schlegel, Mark K.
Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;
GlycoUniverse GmbH & Co. KGaA;

/persons/resource/persons121430

Hurevich, Mattan
Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons121259

Eller, Steffen
Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

/persons/resource/persons128892

Schuhmacher, Frank
Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;
Institute of Chemistry and Biochemistry, Free University Berlin;

/persons/resource/persons85131

Hofmann, Johanna
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institute of Chemistry and Biochemistry, Free University Berlin;

/persons/resource/persons32738

Pagel, Kevin
Molecular Physics, Fritz Haber Institute, Max Planck Society;
Institute of Chemistry and Biochemistry, Free University Berlin;

/persons/resource/persons121849

Seeberger, Peter H.
Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;
Institute of Chemistry and Biochemistry, Free University Berlin;

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Citation

Hahm, H. S., Schlegel, M. K., Hurevich, M., Eller, S., Schuhmacher, F., Hofmann, J., et al. (2017). Automated glycan assembly using the Glyconeer 2.1 synthesizer. Proceedings of the National Academy of Sciences of the United States of America, 114(17), E3385-E3389. doi:10.1073/pnas.1700141114.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-3E90-E

Abstract

Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure–activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydrate-based vaccines.