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Journal Article

Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish

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Portugues,  Ruben
Max Planck Research Group: Sensorimotor Control / Portugues, MPI of Neurobiology, Max Planck Society;

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Citation

Orger, M. B., & Portugues, R. (2016). Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish. Methods in Molecular Biology, 1451, 307-320. doi:10.1007/978-1-4939-3771-4_21.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-A431-0
Abstract
We present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data.