Structural basis of RNA polymerase I transcription initiation.

Engel, C.; Gubbey, T.; Neyer, S.; Sainsbury, S.; Oberthür, C.; Bäjen, C.; Bernecky, C.; Cramer, P.

doi:10.1016/j.cell.2017.03.003

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Structural basis of RNA polymerase I transcription initiation.

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Engel, C.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Gubbey, T.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Neyer, S.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Sainsbury, S.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Oberthür, C.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Bäjen, C.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Bernecky, C.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Cramer, P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Zitation

Engel, C., Gubbey, T., Neyer, S., Sainsbury, S., Oberthür, C., Bäjen, C., et al. (2017). Structural basis of RNA polymerase I transcription initiation. Cell, 169(1), 120-131. doi:10.1016/j.cell.2017.03.003.

Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002C-DD12-C

Zusammenfassung

Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.