English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Using the Volta phase plate with defocus for cryo-EM single particle analysis.

MPS-Authors
/persons/resource/persons123013

Danev,  Radostin
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons203229

Tegunov,  Dmitry
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons77721

Baumeister,  Wolfgang
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

e23006-download.pdf
(Publisher version), 3MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Danev, R., Tegunov, D., & Baumeister, W. (2017). Using the Volta phase plate with defocus for cryo-EM single particle analysis. eLife, 6: e23006. doi:10.7554/eLife.23006.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002C-9FB4-3
Abstract
Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases the data acquisition speed. It also removes the resolution limit inherent to the in-focus method thus allowing 3D reconstructions with resolutions better than 3 angstrom.