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Journal Article

31P-NMR spectroscopy of phosphate compartmentation during ischaemia in hearts protected by cardioplegic treatment.

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Stier,  A.
Department of Spectroscopy and Photochemical Kinetics, MPI for biophysical chemistry, Max Planck Society;

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Finch,  S.A.E.
Department of Spectroscopy and Photochemical Kinetics, MPI for biophysical chemistry, Max Planck Society;

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2403198.pdf
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Citation

Stier, A., Finch, S., Schäfer, H., Gebhardt, M. M., & Bretschneider, H. J. (1989). 31P-NMR spectroscopy of phosphate compartmentation during ischaemia in hearts protected by cardioplegic treatment. Free Radical Research Communications, 7(3-6), 293-300. doi:10.3109/10715768909087954.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002C-8A43-F
Abstract
Four tissue compartments, differing in proton and inorganic phosphate concentration, were resolved by 31P-NMR spectroscopy in samples from dog hearts after cardioplegic treatment with HTK solution. Inversion of the physiological cytoplasmic-mitochondrial pH gradient was observed. The considerable ensuing acidosis of the matrix is discussed with regard to a possible delocalisation of ferrous ions.