Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Metaproteomic analysis of atmospheric aerosol samples

MPG-Autoren
/persons/resource/persons187664

Liu,  Fobang
Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society;

/persons/resource/persons101093

Lai,  S. C.
Atmospheric Chemistry, Max Planck Institute for Chemistry, Max Planck Society;

/persons/resource/persons140324

Reinmuth-Selzle,  K.
Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society;

/persons/resource/persons199007

Scheel,  J. F.
Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society;

/persons/resource/persons100944

Fröhlich-Nowoisky,  J.
Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society;

/persons/resource/persons101189

Pöschl,  U.
Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society;

/persons/resource/persons140326

Kampf,  C. J.
Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society;

Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte in PuRe verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Liu, F., Lai, S. C., Reinmuth-Selzle, K., Scheel, J. F., Fröhlich-Nowoisky, J., Despres, V. R., et al. (2016). Metaproteomic analysis of atmospheric aerosol samples. Analytical and Bioanalytical Chemistry, 408(23), 6337-6348. doi:10.1007/s00216-016-9747-x.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002C-E727-B
Zusammenfassung
Metaproteomic analysis of air particulate matter provides information about the abundance and properties of bioaerosols in the atmosphere and their influence on climate and public health. We developed and applied efficient methods for the extraction and analysis of proteins from glass fiber filter samples of total, coarse, and fine particulate matter. Size exclusion chromatography was applied to remove matrix components, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied for protein fractionation according to molecular size, followed by in-gel digestion and LC-MS/MS analysis of peptides using a hybrid Quadrupole-Orbitrap MS. Maxquant software and the Swiss-Prot database were used for protein identification. In samples collected at a suburban location in central Europe, we found proteins that originated mainly from plants, fungi, and bacteria, which constitute a major fraction of primary biological aerosol particles (PBAP) in the atmosphere. Allergenic proteins were found in coarse and fine particle samples, and indications for atmospheric degradation of proteins were observed.