English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors

MPS-Authors
/persons/resource/persons131138

Falk,  Sebastian
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons193033

Finogenova,  Ksenia
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons77736

Benda,  Christian
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons77867

Conti,  Elena
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

ncomms13573.pdf
(Publisher version), 2MB

Supplementary Material (public)

ncomms13573-s1.pdf
(Supplementary material), 2MB

ncomms13573-s2.pdf
(Supplementary material), 47KB

Citation

Falk, S., Finogenova, K., Melko, M., Benda, C., Lykke-Andersen, S., Jensen, T. H., et al. (2016). Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors. Nature Communications, 7: 13573. doi:10.1038/ncomms13573.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002C-5D4B-4
Abstract
The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. Here we elucidate how RBM7 is incorporated in the NEXT complex. We identify a proline-rich segment of ZCCHC8 as the interaction site for the RNA-recognition motif (RRM) of RBM7 and present the crystal structure of the corresponding complex at 2.0 resolution. On the basis of the structure, we identify a proline-rich segment within the splicing factor SAP145 with strong similarity to ZCCHC8. We show that this segment of SAP145 not only binds the RRM region of another splicing factor SAP49 but also the RRM of RBM7. These dual interactions of RBM7 with the exosome and the spliceosome suggest a model whereby NEXT might recruit the exosome to degrade intronic RNAs.