Crystallization and preliminary X-ray analysis of UMP/CMP-kinase from 
Dictyostelium discoideum with the specific bisubstrate inhibitor P1-(adenosine 
5′)-P5-(uridine 5′)-pentaphosphate (UP5A)

Wiesmüller, Lisa; Scheffzek, Klaus; Kliche, Werner; Goody, Roger S.; Wittinghofer, Alfred; Reinstein, Jochen

doi:10.1016/0014-5793(95)00271-A

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Crystallization and preliminary X-ray analysis of UMP/CMP-kinase from Dictyostelium discoideum with the specific bisubstrate inhibitor P1-(adenosine 5′)-P5-(uridine 5′)-pentaphosphate (UP5A)

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Wiesmüller, Lisa
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Scheffzek, Klaus
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Kliche, Werner
Emeritus Group Bioorganic Chemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Goody, Roger S.
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Wittinghofer, Alfred
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Reinstein, Jochen
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Wiesmüller, L., Scheffzek, K., Kliche, W., Goody, R. S., Wittinghofer, A., & Reinstein, J. (1995). Crystallization and preliminary X-ray analysis of UMP/CMP-kinase from Dictyostelium discoideum with the specific bisubstrate inhibitor P1-(adenosine 5′)-P5-(uridine 5′)-pentaphosphate (UP5A). FEBS Letters, 363(1), 22-24. doi:10.1016/0014-5793(95)00271-A.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002C-4231-8

Abstract

UMP/ICMP-kinase (UK) from the slime mold Dictyostelium discoideum has been purified to high homogeneity and co-crystallized with the bisubstrate inhibitor P1-(adenosine 5′)-P5-(uridine 5′)-pentaphosphate (UP5A). UP5A binds to UK with a dissociation constant (K d ) of 3 ± 0.5 nM at 25°C and pH 7.5. This is some 50-fold tighter than the binding of P1,P5-(diadenosine 5′)-pentaphosphate (AP5A, K d = 160 ± 15 nM). AP5A is a bisubstrate inhibitor that is specific for adenylate kinase. The crystals have the symmetry of the tetragonal space group P41212 or its enantiomorph P43212. The unit cell dimensions are math formula. The crystals diffract to a Bragg spacing of 2.1 Å.