Challenges in imaging cell surface receptor clusters

Medda, Rebecca; Giske, Arnold; Cavalcanti-Adam, Elisabetta Ada

doi:10.1016/j.optlaseng.2015.03.020

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Challenges in imaging cell surface receptor clusters

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Medda, Rebecca
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Cavalcanti-Adam, Elisabetta Ada
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Citation

Medda, R., Giske, A., & Cavalcanti-Adam, E. A. (2016). Challenges in imaging cell surface receptor clusters. Optics and Lasers in Engineering, 76, 3-8. doi:10.1016/j.optlaseng.2015.03.020.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0029-ACEB-3

Abstract

Super-resolution microscopy offers unique tools for visualizing and resolving cellular structures at the molecular level. STED microscopy is a purely optical method where neither complex sample preparation nor mathematical post-processing is required. Here we present the use of STED microscopy for imaging receptor cluster composition. We use two-color STED to further determine the distribution of two different receptor subunits of the family of receptor serine/threonine kinases in the presence or absence of their ligands. The implications of receptor clustering on the downstream signaling are discussed, and future challenges are also presented.