Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers

MPG-Autoren
/persons/resource/persons121172

Brezesinski,  Gerald
Gerald Brezesinski, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte in PuRe verfügbar
Ergänzendes Material (frei zugänglich)

2346779_supp.pdf
(Ergänzendes Material), 119KB

Zitation

Wojciechowski, K., Orczyk, M., Gutberlet, T., Brezesinski, G., Geue, T., & Fontaine, P. (2016). On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers. Langmuir, 32(35), 9064-9073. doi:10.1021/acs.langmuir.6b01737.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002B-6074-D
Zusammenfassung
In this article, we describe the effect of a highly hemolytic saponin, digitonin, on model lipids cholesterol and dipalmitoylphosphatidylcholine (DPPC) using a combination of tensiometric (surface pressure and dilatational surface elasticity), spectroscopic (infrared reflection absorption spectroscopy, IRRAS), microscopic (fluorescence microscopy), and scattering techniques (neutron reflectivity, NR, and grazing incidence X-ray diffraction, GIXD). The monolayers of individual lipids and their 10:9 (mol/mol) mixture were exposed to an aqueous solution of digitonin (10–4 M) by subphase exchange using a setup developed recently in our laboratory. The results confirm that digitonin can adsorb onto both bare and lipid-covered water–air interfaces. In the case of DPPC, a relatively weak interaction can be observed, but the presence of cholesterol drastically enhances the effect of digitonin. The latter is shown to dissociate the weak cholesterol–DPPC complexes and to bind cholesterol in an additional layer attached to the original lipid monolayer.