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Journal Article

Development of insect resistance in tomato plants expressing the δ-endotoxin gene of Bacillus thuringiensis subsp. tenebrionis

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Geider,  Klaus
Research Group Prof. Dr. Geider, Max Planck Institute for Medical Research, Max Planck Society;

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http://link.springer.com/content/pdf/10.1007%2FBF02277423.pdf
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https://dx.doi.org/10.1007/BF02277423
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Citation

Rhim, S.-L., Cho, H.-J., Kim, B.-D., Schnetter, W., & Geider, K. (1995). Development of insect resistance in tomato plants expressing the δ-endotoxin gene of Bacillus thuringiensis subsp. tenebrionis. Molecular Breeding, 1(3), 229-236. doi:10.1007/BF02277423.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-53A4-0
Abstract
A crystal δ-endotoxin gene of Bacillus thuringiensis subsp. tenebrionis (B.t.t.) encoding a coleopteran insect-specific toxin was used to construct a chimeric gene which expressed the toxin in plant cells. Via an Agrobacterium tumefaciens binary vector system, the toxin gene was transferred into tomato cells. From leaf disks recombinant plants were regenerated. Hybridization experiments demonstrated that these plants synthesized toxin-specific mRNA of the expected size. Transgenic tomato plants with the chimeric B.t.t. toxin gene contained a 74 kDa protein which cross-reacted with toxin antibodies. The expression caused a significant insecticidal activity of the transgenic tomato plants against Colorado potato beetle larvae.