Fluorogenic probes for multicolor imaging in living cells.

Lukinavicius, G.; Reymond, L.; Umezawa, K.; Sallin, O.; D'Este, E.; Göttfert, F.; Ta, H.; Hell, S. W.; Urano, Y.; Johnsson, K.

doi:10.1021/jacs.6b04782

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Fluorogenic probes for multicolor imaging in living cells.

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Lukinavicius, G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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D'Este, E.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Göttfert, F.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Ta, H.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Hell, S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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http://pubs.acs.org/doi/full/10.1021/jacs.6b04782
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Zitation

Lukinavicius, G., Reymond, L., Umezawa, K., Sallin, O., D'Este, E., Göttfert, F., et al. (2016). Fluorogenic probes for multicolor imaging in living cells. Journal of the American Chemical Society, 138(30), 9365-9368. doi:10.1021/jacs.6b04782.

Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002B-3421-A

Zusammenfassung

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.