Comment on "Extended-resolution structured illumination imaging of endocytic 
and cytoskeletal dynamics"

Sahl, S.; Balzarotti, F.; Keller-Findeisen, J.; Leutenegger, M.; Westphal, V.; Egner, A.; Lavoie-Cardinal, F.; Chmyrov, A.; Grotjohann, T.; Jakobs, S.

doi:10.1126/science.aad7983

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Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics"

MPG-Autoren

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Sahl, S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Balzarotti, F.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons188143

Keller-Findeisen, J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15441

Leutenegger, M.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons16012

Westphal, V.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15026

Egner, A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons39415

Lavoie-Cardinal, F.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons98970

Chmyrov, A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons39409

Grotjohann, T.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15269

Jakobs, S.
Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society;

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http://science.sciencemag.org/content/352/6285/527.1.full
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Zitation

Sahl, S., Balzarotti, F., Keller-Findeisen, J., Leutenegger, M., Westphal, V., Egner, A., et al. (2016). Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics". Science, 352(6285): 527a. doi:10.1126/science.aad7983.

Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002A-4FAF-0

Zusammenfassung

Li et al. (Research Articles, 28 August 2015, aab3500) purport to present solutions to long-standing challenges in live-cell microscopy, reporting relatively fast acquisition times in conjunction with improved image resolution. We question the methods’ reliability to visualize specimen features at sub–100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features. We are also concerned about the chosen approach of subjectively comparing images from different super-resolution methods, as opposed to using quantitative measures.