Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED 
microscopy in living cells.

Butkevich, A.; Mitronova, G.; Sidenstein, S.; Klocke, J.; Kamin, D.; Meineke, D. N. H.; D'Este, E.; Kraemer, P. T.; Danzl, J. G.; Belov, V. N.; Hell, S. W.

doi:10.1002/anie.201511018

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Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells.

MPG-Autoren

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Butkevich, A.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Mitronova, G.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons189436

Sidenstein, S.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons191593

Klocke, J.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons85147

Kamin, D.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons123288

Meineke, D. N. H.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons98972

D'Este, E.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons191597

Kraemer, P. T.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons73479

Danzl, J. G.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons14832

Belov, V. N.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15210

Hell, S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Zitation

Butkevich, A., Mitronova, G., Sidenstein, S., Klocke, J., Kamin, D., Meineke, D. N. H., et al. (2016). Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells. Angewandte Chemie International Edition, 55(10), 3290-3294. doi:10.1002/anie.201511018.

Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002A-041D-0

Zusammenfassung

A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm.