Nanobodies: Site-specific labeling for super-resolution imaging, rapid 
epitope-mapping and native protein complex isolation.

Pleiner, T.; Bates, M.; Trakhanov, S.; Lee, C. T.; Schliep, J. E.; Chug, H.; Böhning, M.; Stark, H.; Urlaub, H.; Görlich, D.

doi:10.7554/eLife.11349

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Nanobodies: Site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation.

MPS-Authors

/persons/resource/persons135005

Pleiner, T.
Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons36457

Bates, M.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons181080

Trakhanov, S.
Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons188023

Lee, C. T.
Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons188187

Schliep, J. E.
Department of Structural Dynamics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons181078

Chug, H.
Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons188185

Böhning, M.
Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15857

Stark, H.
Department of Structural Dynamics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15947

Urlaub, H.
Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15132

Görlich, D.
Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society;

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引用

Pleiner, T., Bates, M., Trakhanov, S., Lee, C. T., Schliep, J. E., Chug, H., Böhning, M., Stark, H., Urlaub, H., & Görlich, D. (2015). Nanobodies: Site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. eLife, 4:. doi:10.7554/eLife.11349.

引用: https://hdl.handle.net/11858/00-001M-0000-0029-535B-B

要旨

Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to locate individual NPC proteins with.