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Book Chapter

Analyzing protein-phosphoinositide interactions with liposome flotation assays.

MPS-Authors
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Busse,  R. A.
Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society;

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Scacioc,  A.
Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society;

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Schalk,  A. M.
Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society;

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Kühnel,  K.
Research Group of Autophagy, MPI for Biophysical Chemistry, Max Planck Society;

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http://link.springer.com/content/pdf/10.1007%2F978-1-4939-3170-5_13.pdf
(Publisher version)

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Citation

Busse, R. A., Scacioc, A., Schalk, A. M., Krick, R., Thumm, M., & Kühnel, K. (2016). Analyzing protein-phosphoinositide interactions with liposome flotation assays. In M. Waugh (Ed.), Lipid signaling protocols (2. ed., pp. 155-162). New York, N.Y.: Springer. doi:10.1007/978-1-4939-3170-5_13.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-4652-8
Abstract
Liposome flotation assays are a convenient tool to study protein-phosphoinositide interactions. Working with liposomes resembles physiological conditions more than protein-lipid overlay assays, which makes this method less prone to detect false positive interactions. However, liposome lipid composition must be well-considered in order to prevent nonspecific binding of the protein through electrostatic interactions with negatively charged lipids like phosphatidylserine. In this protocol we use the PROPPIN Hsv2 (homologous with swollen vacuole phenotype 2) as an example to demonstrate the influence of liposome lipid composition on binding and show how phosphoinositide binding specificities of a protein can be characterized with this method.