Fourier transform infrared spectroscopic characterization of a photolabile 
precursor of glutamate

Jayaraman, Vasanthi; Thiran, Shalita; Madden, Dean R.

doi:10.1016/S0014-5793(00)01690-2

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Fourier transform infrared spectroscopic characterization of a photolabile precursor of glutamate

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Madden, Dean R.
Max Planck Research Group Ion Channel Structure (Dean R. Madden), Max Planck Institute for Medical Research, Max Planck Society;

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http://www.sciencedirect.com/science/article/pii/S0014579300016902/pdf?md5=8c2fa9261c2fa52cd54fa53c93435e5c&pid=1-s2.0-S0014579300016902-main.pdf
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Citation

Jayaraman, V., Thiran, S., & Madden, D. R. (2000). Fourier transform infrared spectroscopic characterization of a photolabile precursor of glutamate. FEBS Letters, 475(3), 278-282. doi:10.1016/S0014-5793(00)01690-2.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-34EB-1

Abstract

Recently, it has been demonstrated that Fourier transform infrared spectroscopy (FTIR) detects conformational changes in the glutamate receptor ligand-binding domain that are associated with agonist binding. Combined with flash photolysis, this observation offers the prospect of following conformational changes at individual protein and agonist moieties in parallel and with high temporal resolution. Here, we demonstrate that small gamma, Greek(small alpha, Greek-carboxy-2-nitrobenzyl) glutamate (caged glutamate) does not interact with the protein, and that following photolysis with UV light the FTIR difference spectrum indicated changes in the protein tertiary and secondary interactions. These changes were similar to those observed for the protein upon addition of free glutamate. Thus, caged glutamate and its photolysis by-products are inert in this system, whereas the released glutamate exhibits full activity. Difference spectra of caged glutamate and of reaction analogs permitted identification of and correction for FTIR signals arising from the photolytic reaction and confirmed that its products are indeed glutamate and 2-nitrosophenyl glyoxalic acid