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Journal Article

Sequential backbone assignment based on dipolar amide-to-amide correlation experiments.

MPS-Authors
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Xiang,  S. Q.
Research Group of Solid-State NMR-2, MPI for Biophysical Chemistry, Max Planck Society;

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Grohe,  K.
Research Group of Solid-State NMR-2, MPI for Biophysical Chemistry, Max Planck Society;

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Rovo,  P.
Research Group of Solid-State NMR-2, MPI for Biophysical Chemistry, Max Planck Society;

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Vasa,  S. K.
Research Group of Solid-State NMR-2, MPI for Biophysical Chemistry, Max Planck Society;

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Giller,  K.
Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Becker,  S.
Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Linser,  R.
Research Group of Solid-State NMR-2, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

http://link.springer.com/content/pdf/10.1007%2Fs10858-015-9945-4.pdf
(Publisher version)

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Supplementary Material (public)

2176343_Suppl.pdf
(Supplementary material), 5MB

Citation

Xiang, S. Q., Grohe, K., Rovo, P., Vasa, S. K., Giller, K., Becker, S., et al. (2015). Sequential backbone assignment based on dipolar amide-to-amide correlation experiments. Journal of Biomolecular NMR, 62(3), 303-311. doi:10.1007/s10858-015-9945-4.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-1A33-B
Abstract
Proton detection in solid-state NMR has seen a tremendous increase in popularity in the last years. New experimental techniques allow to exploit protons as an additional source of information on structure, dynamics, and protein interactions with their surroundings. In addition, sensitivity is mostly improved and ambiguity in assignment experiments reduced. We show here that, in the solid state, sequential amide-to-amide correlations turn out to be an excellent, complementary way to exploit amide shifts for unambiguous backbone assignment. For a general assessment, we compare amide-to-amide experiments with the more common C-13-shift-based methods. Exploiting efficient CP magnetization transfers rather than less efficient INEPT periods, our results suggest that the approach is very feasible for solid-state NMR.