Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Lentivirus−based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo

MPG-Autoren
/persons/resource/persons92679

Dittgen,  Tanjew
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94557

Nimmerjahn,  Axel
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons118198

Komai,  Shoji
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94079

Licznerski,  Pawel
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95861

Waters,  David Jack
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94233

Margrie,  Troy W.
Max Planck Research Group Behavioural Neurophysiology (Andreas T. Schaefer), Max Planck Institute for Medical Research, Max Planck Society;
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons93373

Helmchen,  Fritjof
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons128986

Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94635

Osten,  Pavel
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte in PuRe verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Dittgen, T., Nimmerjahn, A., Komai, S., Licznerski, P., Waters, D. J., Margrie, T. W., et al. (2004). Lentivirus−based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Proceedings of the National Academy of Sciences of the United States of America, 101(52), 18206-18211. doi:10.1073/pnas.0407976101.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0019-9C59-7
Zusammenfassung
It is becoming increasingly clear that single cortical neurons encode complex and behaviorally relevant signals, but efficient means to study gene functions in small networks and single neurons in vivo are still lacking. Here, we establish a method for genetic manipulation and subsequent phenotypic analysis of individual cortical neurons in vivo. First, lentiviral vectors are used for neuron−specific gene delivery from {alpha}−calcium/calmodulin−dependent protein kinase II or Synapsin I promoters, optionally in combination with gene knockdown by means of U6 promoter−driven expression of short−interfering RNAs. Second, the phenotypic analysis at the level of single cortical cells is carried out by using two−photon microscopy−based techniques: high−resolution two−photon time−lapse imaging is used to monitor structural dynamics of dendritic spines and axonal projections, whereas cellular response properties are analyzed electrophysiologically by two−photon microscopydirected whole−cell recordings. This approach is ideally suited for analysis of gene functions in individual neurons in the intact brain