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Visualization of LC-MS/MS proteomics data in MaxQuant

MPG-Autoren
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Tyanova,  Stefka
Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Temu,  Tikira
Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Carlson,  Arthur
Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Sinitcyn,  Pavel
Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Mann,  Matthias
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Cox,  Juergen
Cox, Jürgen / Computational Systems Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Zitation

Tyanova, S., Temu, T., Carlson, A., Sinitcyn, P., Mann, M., & Cox, J. (2015). Visualization of LC-MS/MS proteomics data in MaxQuant. PROTEOMICS, 15(8), 1453-1456. doi:10.1002/pmic.201400449.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0026-CB52-D
Zusammenfassung
Modern software platforms enable the analysis of shotgun proteomics data in an automated fashion resulting in high quality identification and quantification results. Additional understanding of the underlying data can be gained with the help of advanced visualization tools that allow for easy navigation through large LC-MS/MS datasets potentially consisting of terabytes of raw data. The updated MaxQuant version has a map navigation component that steers the users through mass and retention time-dependent mass spectrometric signals. It can be used to monitor a peptide feature used in label-free quantification over many LC-MS runs and visualize it with advanced 3D graphic models. An expert annotation system aids the interpretation of the MS/MS spectra used for the identification of these peptide features.