High speed optical coherence microscopy with autofocus adjustment and a 
miniaturized endoscopic imaging probe

Aguirre, Aaron D.; Sawinski, Jürgen; Huang, Shu Wei; Zhou, Chao; Denk, Winfried; Fujimoto, James G.

doi:10.1364/OE.18.004222

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High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe

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Sawinski, Jürgen
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Denk, Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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http://dx.doi.org/10.1364/OE.18.004222
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Citation

Aguirre, A. D., Sawinski, J., Huang, S. W., Zhou, C., Denk, W., & Fujimoto, J. G. (2010). High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe. Optics Express, 18(5), 4222-4239. doi:10.1364/OE.18.004222.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-2092-C

Abstract

Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high−speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro−optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto−focusing feature that enables precise, near real−time alignment of the confocal and coherence gates in tissue, allowing user−friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber−scanning probe which can be adapted for laparoscopic and endoscopic imaging applications