Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional 
RNA.

Wawrzyniak-Turek, K.; Höbartner, C.

doi:10.1039/C4CC04719B

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Journal Article

Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA.

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Wawrzyniak-Turek, K.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

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Höbartner, C.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

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Citation

Wawrzyniak-Turek, K., & Höbartner, C. (2014). Enzymatic combinatorial nucleoside deletion scanning mutagenesis of functional RNA. Chemical Communications, 50(75), 10937-10940. doi:10.1039/C4CC04719B.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0023-D2A8-2

Abstract

We describe a general and simple method to identify catalytically and structurally important nucleotides in functional RNAs. Our approach is based on statistical replacement of each nucleoside with a non-nucleosidic spacer (C3 linker, Δ), followed by separation of active library variants and readout of interference effects by analysis of enzymatic primer extension reactions.